521 Development of 3D reconstructed skin models with PBMC-derived immune cells

Skin immune system is composed of specialized cells that plays an important role in skin homeostasis and protection against injury. Among these cells, Langerhans macrophages are key elements both the innate adaptive response. In epidermal compartment, (LCs) immature dendritic involved antigen presentation to T Cells whereas dermal tissue resident professional phagocytes. Under inflammatory context, epidermis dermis depleted from through migration or cell death respectively. Attracted site by cytokine signals, they differentiate into Macrophages Dendritic depending on chemical environment. this study, we took advantage mechanism generate type 1 (M1) 2 (M2) Peripheral Blood Mononuclear (PBMC) derived monocytes. Then, were integrated new engineered human models for vitro testing. M1 M2 incorporated equivalent alone full thickness (Dermis + epidermis) thickness. These 3D reconstructions allowed study morphology expression specific markers order delineate each subpopulations. immunocompetent aimed restore physiological interplay between main types: keratinocytes/ fibroblasts. Indeed, promising tools monitoring following biofunctional ingredients chemicals application.